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What is Holotranscobalamin?

the active form of B12

Holotranscobalamin measures the biologically active part of B12 - the fraction that cells can actually take up. It is a more sensitive measure than total B12.

Only about 20 percent of blood B12 is active. Holo-TC measures this fraction and is useful in borderline cases where total B12 is near the threshold.

Test facts

Unit
pmol/L
Sample
Serum
Fasting
No fasting needed
Turnaround
3 days

What does low Holotranscobalamin mean?

Low holo-TC points to functional B12 deficiency even when total B12 is normal.

What does high Holotranscobalamin mean?

High holo-TC is not clinically problematic.

Panels that measure Holotranscobalamin

  • Vitamins & Minerals Full

Launching in Stockholm in 2026.

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This is general information about the marker, not an assessment of your values and not medical advice. Last updated: May 2026.